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Feb 12, 2008 · VLR4-containing supernatant was passed over the BclA-1-island column, which was washed with PBS before elution of VLR-B with 0.1 M triethylamine, pH 11.5, into a tube containing 0.5 ml of 1 M Tris, pH 6.8, dialysis into 10 mM Hepes, 150 mM NaCl, 0.02% sodium azide, pH 7.2, and concentration.
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